Alleles' Frequency Distribution of Two Str Loci in Egyptian Population By

Deoxyribonucleic acid (DNA) typing is widely used for personal identification. The allele frequency distribution of two STR loci (CSF1PO and TPOX) was studied in Egyptian population. A sample of 100 unrelated individuals inhabiting Dakahlia governorate (in Delta region) in Egypt was chosen randomly. Blood samples were collected after obtaining an informed consent. The DNA was extracted by using the standard phenol-chloroform extraction method and then amplified using the HYBAID Thermal Cycler. Four alleles were identified at each locus with frequencies 0.34 to 0.18 for CSF1PO locus and 0.37 to 0.085 for TPOX locus. By studying MP, PE, PD, PIC and TPI parameters; CSF1PO locus has the highest MP, PE and TPI values (0.227, 0.033 and 0.63 respectively). Meanwhile PD and PIC have the highest values in TPOX locus (0.807 and 0.69 respectively). It is concluded that the presence of certain alleles and absence of others only in the studied population compared to other presented studies on Egyptians, Arabs and Israeli populations can be specific. TPOX locus was found to be highly polymorphic and could be used in forensic investigations and issue of identity. Meanwhile both CSF1PO and TPOX loci are less informative in paternity testing.